Role of SIRT1 in Hepatic Encephalopathy: In Vivo and In Vitro Studies Focusing on the NLRP3 Inflammasome

Figure 6

EX527 enhanced the activation of NLRP3 inflammasome in BV2 microglial cells. (a) The cell viability of BV2 cells at different concentrations EX527; (b) BV2 microglial cells exposed to EX527 (5, 10, 20, and 40 μM) for 8 h; then, the protein expression of SIRT1 was measured by western blotting; (c) the quantitative blots of SIRT1; (d) cells were exposed to normal saline, 10 μg/ml LPS+4 mM ATP, 10 μg/ml LPS+4 mM ATP+40 μM EX527, and 40 μM EX527. The protein expression of SIRT1, NLRP3, ASC, and IL-1β was detected by western blotting; (e) the quantitative blots of SIRT1; (f) the quantitative blots of NLRP3, ASC, and IL-1β. , compared with the control group; ^#, compared with the control+10 μg/ml LPS+4 mM ATP group. per group. The data represent the .

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