Supplemental N-3 Polyunsaturated Fatty Acids Limit A1-Specific Astrocyte Polarization via Attenuating Mitochondrial Dysfunction in Ischemic Stroke in Mice

Figure 4

Effects of DHA on mitophagy in the astrocytes treated with hypoxia. Primary astrocytes were cultured at 21.0% or 1.0% oxygen in the presence or absence of DHA. (a) The expression of parkin, p62, and LC3 in the astrocytes was determined by Western blot analysis. The expressions of P62 and LC3 in brain tissue (b) and primary astrocytes (c) are shown by immunofluorescence staining. (d) The expression of mito-pink1 and mito-parkin in the mitochondrial fractions was determined by Western blot analysis. (e) The expression of mfn1 and mfn2 was detected by immunoblotting. Quantification was performed in images using the Image J software. . Data from one representative experiment of three independent experiments are presented. Scale bar 100 μm in (b), scale bar 20 μm in (c).

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