Protective Effect of Penetratin Analogue-Tagged SOD1 on Cisplatin-Induced Nephrotoxicity through Inhibiting Oxidative Stress and JNK/p38 MAPK Signaling Pathway

Figure 1

Screened PEN-SOD1 can efficiently penetrate the cell membrane. (a) 11 CPPs-SOD1 were expressed, purified, and detected by Coomassie brilliant blue staining (upper panel) and Western blot (lower panel). (b) SOD enzyme activity of 11 CPPs-SOD1 was measured according to the manufacturer’s instructions. (c-e) VERO and HK-2 cells were treated with 4 μM CPPs-SOD1 for 1 h. Cell membrane penetrating ability of 11 CPPs-SOD1 was determined by immunofluorescence staining with SOD1 (green) and Hoechst (blue) (c) and further analyzed by Western blot (d, e).

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