Protective Effect of Penetratin Analogue-Tagged SOD1 on Cisplatin-Induced Nephrotoxicity through Inhibiting Oxidative Stress and JNK/p38 MAPK Signaling Pathway

Figure 3

PSF-SOD1 increases intracellular total SOD enzyme activity. VERO and HK-2 cells were treated with 1, 2, 4 μM PSF-SOD1 and 1, 2, 4 μM TAT-SOD1 for 60 min or 4 μM PSF-SOD1 and 4 μM TAT-SOD1 for 10, 30, and 60 min. (a, b) Western blot analysis with SOD1 antibody in cell lysates. (c, d) Intracellular SOD enzyme activity in cell lysates was measured by SOD assay kit. ^##, ^### compared with control cells. , , compared with TAT-SOD1-treated cells.

(a)

(b)

(c)

(d)