Rosiglitazone Suppresses Renal Crystal Deposition by Ameliorating Tubular Injury Resulted from Oxidative Stress and Inflammatory Response via Promoting the Nrf2/HO-1 Pathway and Shifting Macrophage Polarization

Figure 2

ROSI decreased renal tubular injury, cell apoptosis, and proinflammatory response in the mouse model. (a) PAS staining. PAS staining denotes tubular injury (arrows). Scale . (b) Cell apoptosis in the kidneys (arrows). Scale . (c) The percentage of damaged tubules displayed in PAS staining. (d) The mean number of apoptotic cells per high-power field (×400; fields per section) in the TUNEL assay. (e) Immunohistochemical distribution of genetic expression of PPARγ, Mps-related molecule MCP1, and proinflammatory cytokine IL-1β. Scale . (f) The proportion of the IHC-positive area. Gly: glyoxylic acid; ROSI: rosiglitazone; PAS: periodic acid–Schiff; IL-1β: interleukin-1β; MCP1: monocyte chemotactic protein-1; PPARγ: peroxisome proliferator-activated receptor γ; IHC: immunohistochemistry. ; .

(a)

(b)

(c)

(d)

(e)

(f)