Research Article
Prunus mume Seed Exhibits Inhibitory Effect on Skin Senescence via SIRT1 and MMP-1 Regulation
Figure 6
Effect of P. mume ripened seed (PmRS) treatment on mitogen-activated protein kinase (MAPK) signaling in HaCaT cells. The effect of PmRS (10, 30, and 100 μg/ml) on cell viability in HaCaT cells (a). Protective effect of PmRS on cell viability (b) and intercellular reactive oxygen species (ROS) generation in ultraviolet- (UV-) B-induced (40 mJ/cm2) HaCaT cells (c). Reverse transcription-polymerase chain reaction (d) and western blot analysis (e) of PmRS on skin photoaging-related markers. Phosphorylation of the MAPK signaling pathway in UVB-induced HaCaT cells (f). NT: no treatment; EGCG: epigallocatechin gallate; PmRS10: PmRS, 10 μg/ml; PmRS30: PmRS, 30 μg/ml; PmRS100: PmRS, 100 μg/ml. Results of RT-PCR and western blots are shown as a classical set of 3 independent experiments.
| (a) |
| (b) |
| (c) |
| (d) |
| (e) |
| (f) |