Exosomal miR-218-5p/miR-363-3p from Endothelial Progenitor Cells Ameliorate Myocardial Infarction by Targeting the p53/JMY Signaling Pathway

Figure 6

p53/JMY as a target of miR-218-5p and miR-363-3p. (a) The predicted binding sites for miR-218-5p in the p53 promoter. (b) Schematic diagram of pGL3-Basic vector construction harboring p53 promoter binding sites. (c) Relative luciferase activity (F/R) assay for miR-218-5p binding to the p53 promoter. cel-miR-39 mimic served as the negative control. The data are presented as the (), , miR-218-5p mimic versus NC or cel-miR-39 mimic. (d) The predicted binding sites for miR-363-3p in the 3UTR of JMY. (e) Schematic diagram of psi-Check2 vector construct harboring the 3UTR of JMY binding sites. (f) Relative luciferase activity (R/F) assay for miR-363-3p in the 3UTR of JMY. The data are presented as the (), , miR-363-3p mimic versus NC or cel-miR-39 mimic. cel-miR-39 mimic served as the negative control. (g) qRT-PCR to detect the relative expression levels of p53 and JMY in CFs in the mimic-Exo and inhibitor-Exo groups. The data are presented as the (). and , mimic-Exo or inhibitor-Exo versus NC-Exo.

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