Immunotherapy Using Oxygenated Water and Tumor-Derived Exosomes Potentiates Antitumor Immune Response and Attenuates Malignancy Tendency in Mice Model of Breast Cancer

Figure 1

Protocol of treatment with oxygenated water and/or exosomes. Ten days after tumor implantation using the 4T1 cell line, the animals were treated with oxygenated water and/or exosomes. Treatment with oxygenated water was performed via the intratumoral and/or intraperitoneal route and was performed on a daily basis. Exosomes were administered via the subcutaneous route every three days. Three days after the last treatment, the animals were euthanized and immunological evaluations were performed.