Phenolic Compounds of Red Wine Aglianico del Vulture Modulate the Functional Activity of Macrophages via Inhibition of NF-κB and the Citrate Pathway

Figure 8

RWP lowered COX2 and PGE₂ levels. Primary human monocytes were activated to macrophages with LPS (LPS) in the absence or in the presence of RWP alone or plus acetate and COX2 (a, b), and PGE₂ levels were quantified. In (a) and (b), COX2 and β-actin proteins were immunodecorated with specific antibodies. The intensities of immunolabeled protein bands were measured by using a quantitative software and normalized to β-actin; values obtained are reported under western blot images. Protein expression levels in control sample were taken as 1, and other samples were expressed in proportion to the control. (c) Following 48 hours, PGE₂ levels were evaluated and expressed as the percentage of the levels in untreated cells (set at 100%). of three replicate independent experiments with three replicates in each are shown. According to one-way ANOVA, differences were significant (). Therefore, Tukey’s post hoc test was performed, and different letters indicate significant differences between treatments at . C: control; L: LPS; R20: RWP 20 μg/mL; R200: RWP 200 μg/mL; Ac: acetate.

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