Exosomal MATN3 of Urine-Derived Stem Cells Ameliorates Intervertebral Disc Degeneration by Antisenescence Effects and Promotes NPC Proliferation and ECM Synthesis by Activating TGF-β

Figure 4

MATN3 was significantly decreased in the nucleus pulposus tissue of intervertebral discs. (a) Proteome analysis of the normal and degenerated discs of humans. The 4 matrilin family proteins were significantly decreased in the degenerated discs. MATN3 was the most differentially expressed (, ). (b, c) WB analysis of MATN3 expression in the normal and degenerated discs of humans. MATN3 significantly decreased in the degenerated discs (, ). (d) Immunofluorescence staining of human nucleus pulposus tissue for MATN3. Blue represents DAPI, and green represents MATN3. (e) Immunofluorescence image of intact rat IVDs for MATN3. (f) Exosome intervention in rat intervertebral discs was associated with the immunofluorescence results of degenerative intervertebral discs.

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