Bmal1 Regulates the Redox Rhythm of HSPB1, and Homooxidized HSPB1 Attenuates the Oxidative Stress Injury of Cardiomyocytes

Figure 4

Circadian rhythm disorder decreases the level of homooxidized HSPB1 and Bmal1 in C57BL/6 mice. (a–d) A piece of ventricular muscle coming from the apex of a mouse heart was extracted at 12, 6, and 24 o’clock (24-hour clock), and two mice were collected from each group each day for a total of 7 days ( for each group). (e–h) The C57BL/6 mice in the experimental group were treated by day-night reversal for 14 days, namely, 6:00 to 18:00 every day as nonlight time (24-hour clock). (a, e) Bmal1 and β-actin were measured by Western blot analysis, and the homooxidized HSPB1 was analyzed by NEM-alkylated redox Western blot. (b, f) HSPB1 redox potentials were determined using band intensities and the Nernst equation. Densitometry was used to determine the fold expression of Bmal1 (c, g) and homooxidized HSPB1 (d, h) compared with β-actin. (a–d) for each group; (e–h) for each group; , , and .

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