UNC5B Promotes Vascular Endothelial Cell Senescence via the ROS-Mediated P53 Pathway

Figure 4

UNC5B induces endothelial cell senescence through ROS production. (a) Young HUVECs were prepared as Figure 3. The production of intracellular ROS was determined with DHE by microscopy. Representative images of stained cultures are shown. (b) Transfection of replicative senescent HUVECs with siRNA for three days. Determination of ROS levels in the cells. Representative images of stained cultures are shown. (c) Once infected with lentiviral vectors, cells were immediately incubated with or without NAC (5 mmol/L). The cells were then stained and analyzed for their SA-β-gal activity. (d) The proliferative potential of these cells treated with or without NAC was detected by Edu incorporation assay. Scale bar, 50 μm. Quantitative data are presented as of three or more independent experiments. means ; means .

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