PPARγ Attenuates Interleukin-1β-Induced Cell Apoptosis by Inhibiting NOX2/ROS/p38MAPK Activation in Osteoarthritis Chondrocytes

Figure 2

Cell viability. (a) Cultured chondrocytes were treated with 0 ng/ml, 1 ng/ml, 10 ng/ml, or 30 ng/ml IL-1β for 0 min, 10 min, 30 min, 1 h, 2 h, 12 h, 24 h, and 48 h, respectively. Cell viability was detected by CCK-8. (b) Cultured chondrocytes were pretreated with 10 mM NAC, 10 μM GW1929, 10 μM DPI, 10 μM SB203580, 10 μM PD98059, or 10 μM SP600125 for 1 h and then treated with 10 ng/ml IL-1β for 0 h, 12 h, 24 h, and 48 h. Cell viability was detected by CCK-8. Results are presented as of three independent experiments. Chondrocytes cultured in DMEM were used as the vehicle control. , , and versus the vehicle control. ^#, ^##, and ^### versus IL-1β group.

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