PPARγ Attenuates Interleukin-1β-Induced Cell Apoptosis by Inhibiting NOX2/ROS/p38MAPK Activation in Osteoarthritis Chondrocytes

Figure 3

Cell apoptosis. (a) Cultured chondrocytes were pretreated with 10 mM NAC, 10 μM GW1929, 10 μM DPI, 10 μM SB203580, 10 μM PD98059, or 10 μM SP600125 for 1 h and then treated with 10 ng/ml IL-1β for 24 h. Chondrocytes cultured in DMEM were used as the vehicle control. FITC annexin V/PI staining and flow cytometry assays were used to detect cell apoptosis. (b) Quantification of apoptosis in different groups. Results are presented as of three independent experiments. and versus the vehicle control. ^## and ^### versus the IL-1β group. ^& and ^&& versus the NAC group.

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