Research Article
Pharmic Activation of PKG2 Alleviates Diabetes-Induced Osteoblast Dysfunction by Suppressing PLCβ1-Ca2+-Mediated Endoplasmic Reticulum Stress
Figure 7
Cinaciguat showed osteoprotective effects via PKG2 by suppressing ER stress under T2DM condition. (a) EDU staining was performed to test the osteoblasts undergoing DNA replication. Red, EDU positive nucleus. Blue, all nucleus. . (b) The quantification analysis of EDU-positive cells. (c) Flow cytometer analysis was conducted to detect cell apoptosis. (d) The quantification analysis of apoptosis rate. (e) Cytoskeleton (red) was stained by rhodamine-phalloidin, and nucleus (blue) was stained by DAPI. . (f) ALP activity assay after 7 days stimulation. (g) ALP staining after 7 days stimulation. (h) The protein levels of COL1, ALP, and RUNX2 were detected by western blot after 7 days stimulation. Data were presented as from at least three independent experiments. : . RP: rhodamine-phalloidin; Cina: cinaciguat; Tm: tunicamycin; 4-PBA: 4-phenylbutyric aci.
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