Research Article
Hypoxia Induced by Cobalt Chloride Triggers Autophagic Apoptosis of Human and Mouse Drug-Resistant Glioblastoma Cells through Targeting the PI3K-AKT-mTOR Signaling Pathway
Figure 2
Effects of hypoxia induced by CoCl2 on levels of hypoxia-inducible factor- (HIF-) 1α and cell survival in human drug-resistant glioblastoma cells. Human drug-tolerant U87 MG-R cells were selected from TMZ-sensitive U87 MG cells. U87 MG-R cells were treated with hypoxia for 6, 12, and 24 h. (a) Levels of HIF-1α were immunodetected (top panel). β-Actin was analyzed as the internal control (bottom panel). (b) These protein bands were quantified and statistically analyzed. (c) Cell morphology was observed and photographed. (d) Cell survival was assayed with a trypan blue exclusion method. Mouse GL261-R glioblastoma cells were exposed to hypoxia for 24 h. (e, f) Levels of HIF-1α and cell survival were analyzed. Data are expressed as the for . vs. control.
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