Ulinastatin Ameliorates IL-1β-Induced Cell Dysfunction in Human Nucleus Pulposus Cells via Nrf2/NF-κB Pathway

Figure 8

Effects of UTI on Nrf2/HO-1 pathway in human NP cells induced by IL-1β. (a, b) Expression at protein level of Nrf2 in nucleus and HO-1 in cytoplasm were analyzed and quantified (). (c) The nuclear translocation of Nrf2 was visualized via immunofluorescence. . (d, e) After knockdown of Nrf2, the protein amount of Nrf2 and p65 in nucleus and iNOS, COX2, c-caspase3, and SOD1 in cytoplasm in NP cells were presented by WB (). (f) Quantification of WB results of Nrf2, p65, iNOS, COX2, c-caspase3, and SOD1. , , , .

(a)

(b)

(c)

(d)

(e)

(f)