The Downregulation of ADAM17 Exerts Protective Effects against Cardiac Fibrosis by Regulating Endoplasmic Reticulum Stress and Mitophagy

Figure 2

ADAM17 knockdown attenuated TGF-β1-induced mCF activation and the upregulation of ECM proteins. (a) The mRNA expression of ADAM17 after three siRNA chain transfections (); siRNA3 was the most efficient chain. (b, c) NC or siRNA3 transfection in the presence or absence of TGF-β1 (5 ng/mL, 48 h). The mRNA and protein levels of ADAM17, collagen I, and α-SMA in activated mCFs after transfection with NC or siRNA3 (). (d) The migration of mCFs (determined by the areas of cells protruding from the wound border) after transduction with NC or siRNA3 (). (e) The proliferation of mCFs was assayed using CCK-8 (). Data in (a–e) are expressed as . indicates , and indicates vs. the NC group; # indicates , and ## indicates vs. the NC+TGF-β1 group.

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