Antitumor Effects of Freeze-Dried Robusta Coffee (Coffea canephora) Extracts on Breast Cancer Cell Lines

Figure 5

Green coffee effect on mitochondrial membrane potential. MDA-MB-231, MCF7, and MCF 10A cells were treated for 0, 4, and 24 h with green coffee extracts at 500 and 1000 μg/mL. Cells were collected and incubated with 100 nM MitoTracker Red CMXRos for 30 min and then analyzed by flow cytometry. Representative histograms of each treatment are presented.