Inhibition of α-Synuclein Accumulation Improves Neuronal Apoptosis and Delayed Postoperative Cognitive Recovery in Aged Mice

Figure 2

Anesthesia plus surgery increased α-syn conformations in mouse brain hippocampus. (a) α-syn-positive granules and pSer129-α-syn were detected by immunostaining in aged (18-month old) mouse hippocampal CA1 and dentate gyrus (DG) subregions at 24 h after surgery. Signal intensity was quantified (a.u.: arbitrary unit). , . (b) Dot blots for total α-syn. pSer129-α-syn was examined by western blotting. Relative levels of total α-syn and pSer129-α-syn are shown (). (c) Accumulation of α-syn oligomers (red) in aged mouse hippocampus was demonstrated by immunostaining using an α-syn oligomer-specific antibody (Syn33). , . (d) pSer129-α-syn was detected by immunostaining in the frontal cortex of 18-month-old mice at 24 h postsurgery. Signal intensity was quantified (a.u.: arbitrary unit). , . Results are presented as (one-way analysis of variance). , , , Control (Con) or anesthesia-alone (Ane) vs. surgery plus anesthesia (Sur).

(a)

(b)

(c)

(d)