Inhibition of α-Synuclein Accumulation Improves Neuronal Apoptosis and Delayed Postoperative Cognitive Recovery in Aged Mice

Figure 4

Clenbuterol attenuated mitochondrial α-syn accumulation in the hippocampus of delayed neurocognitive recovery mice. (a) Representative photomicrographs and statistical analysis of immunohistochemistry of total α-syn and pSer129-α-syn in the hippocampal CA1 and dentate gyrus (DG) subregions of delayed neurocognitive recovery mice. . (b) Protein levels of total α-syn dot blots and pSer129-α-syn western blots in the hippocampus; quantification of relative intensities. (c) Immunofluorescence of α-syn oligomers (Syn33, red) in mouse hippocampus at 24 h after surgery, with mean fluorescence of Syn33. . (d) Immunofluorescence of α-syn (red) in mitochondria (green) of the hippocampus. Arrows point to mitochondrial α-syn, ; /group. Data are expressed as . , , , anesthesia-alone (Ane) vs. surgery plus anesthesia (Sur); ^#, ^##, ^###, surgery plus anesthesia (Sur) vs. surgery plus clenbuterol (Clen+Sur).

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