Melatonin Alleviates the Toxicity of High Nicotinamide Concentrations in Oocytes: Potential Interaction with Nicotinamide Methylation Signaling

Figure 1

Effect of NAM administration on oocyte maturation in presence and absence of melatonin. (a) Light microscopy for the polar body (red arrow) of in vitro matured oocytes. (b) Percentages of oocytes with extruded polar bodies. (c) Microscopy of nuclear maturation using DAPI staining showing the different stages of maturation. (d) Proportion of oocytes that reached the MII stage. (e, f) Filamentous actin distribution in oocytes using Alexa Fluor 488 phalloidin staining. (g) Fluorescence intensity after phalloidin-based staining in oocyte’s cytoplasm and zona pellucida/oolemma.  μm. The error bars represent the SEM of measurements of three replicates. PB: polar body; GV: germinal vesicle; MI: metaphase I; MII: metaphase II. BF: bright field; NAM: nicotinamide; MT: melatonin.

(a)

(b)

(c)

(d)

(e)

(f)

(g)