Melatonin Alleviates the Toxicity of High Nicotinamide Concentrations in Oocytes: Potential Interaction with Nicotinamide Methylation Signaling

Figure 3

Effect of melatonin/NAM supplementation on apoptosis and autophagy in oocytes. (a) The transcription levels of apoptosis-related genes in matured oocytes. (b, c) Representative images of Caspase-3 and Caspase-9 immunofluorescence. (d, e) Fluorescence intensity of Caspase-3 and Caspase-9 in oocytes. (f) Transcriptional pattern of the different autophagy-related markers in oocytes. (g, h) Representative images of Beclin-1 and LC3B staining. (i, j) Fluorescence intensity of Beclin-1 and LC3B in oocytes.  μm. The error bars represent the SEM of measurements of three replicates. BCL2: B-cell lymphoma 2; BAX: Bcl-2-associated X apoptosis regulator; Beclin-1: autophagy-related gene 6; LC3A (MAP1LC3A): microtubule-associated protein 1 light chain 3 alpha; LC3B (MAP1LC3B): microtubule-associated protein 1 light chain 3 beta; ATG7: autophagy-related gene 6; LAMP1: lysosomal-associated membrane protein 1; LAMP2: lysosomal-associated membrane protein 2; NAM: nicotinamide; MT: melatonin.

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