Oxidative Medicine and Cellular Longevity

Research Article

Melatonin Alleviates the Toxicity of High Nicotinamide Concentrations in Oocytes: Potential Interaction with Nicotinamide Methylation Signaling

Table 1

The primers used in RT-qPCR analysis.
Gene nameGene sequenceAccession numberProduct size
Apoptosis-related genes
 Caspase-3F: CCCAAGTGTGACCACTGAAC
R: CCATTAGGCCACACTCACTG		NM_001077840.1169
 BCL2F: TGGATGACCGAGTACCTGAA
R: CAGCCAGGAGAAATCAAACA		NM_001166486.1120
 BAXF: CACCAAGAAGCTGAGCGAGTGT
R: TCGGAAAAAGACCTCTCGGGGA		XM_027515208.1118
Autophagy-related genes
 Beclin-1F: AGTTGAGAAAGGCGAGACAC
R: GATGGAATAGGAACCACCAC		NM_001033627.2100
 MAP1LC3A (LC3A)F: CATGAGCGAGTTGGTCAAAA
R: GGGAGGCGTAGACCATGTAG		XM_027558753.1170
 MAP1LC3B (LC3B)F: TTATCCGAGAGCAGCATCC
R: AGGCTTGATTAGCATTGAGC		NM_001001169.1170
 ATG7F: ATGGCCTTTGAGGAACCTTT
R: ATGCCTCCCTTCTGGTTCTT		XM_010817935.3210
 LAMP1F: GTGAAGAATGGCAACGGAC
R: GCATCAGCTGGACCTCGTAA		XM_027558031.1250
 LAMP2F: AAGAGCAGACCGTTTCCGTG
R: CGAACACTCTTGGGCAGTAG		XM_027535042.1110
Cell cycle and DNA damage-related genes
 P21F: GCAAATATGGGTCTGGGAGA
R: AAATAGTCCAGGCCAGGATG		NM_001098958.2112
 P27F: TGTCAAACGTGCGAGTGTCTA
R: CTCTGCAGTGCTTCTCCAAGT		XM_019961532.1150
 P53F: CTATGAGATGTTCCGAGAGC
R: CTCTCTCTTGAGCATTGGTT		NM_174201.2153
NAM methylation-related genes
 NNMTF: CCCAGGTGCTCAAGTGTGAT
R: CAGCCTCAAGACACAGGGAG		XM_015474625.299
 AHCYF: GCAACTGCTCACTCAGTCCT
R: AGGCCTGGATGGTAAAGTGC		NM_001034315.181
 AOX1F: AATGTGACCCGGAAACTCCC
R: ATGTGGCCCCCTAAAGAAGC		XM_024999354.1116
Reference gene
 GADPHF: CCCAGAATATCATCCCTGCT
R: CTGCTTCACCACCTTCTTGA		NM_001034034.2185