Research Article
Melatonin Alleviates the Toxicity of High Nicotinamide Concentrations in Oocytes: Potential Interaction with Nicotinamide Methylation Signaling
Table 1
The primers used in RT-qPCR analysis.
| Gene name | Gene sequence | Accession number | Product size |
| Apoptosis-related genes | Caspase-3 | F: CCCAAGTGTGACCACTGAAC R: CCATTAGGCCACACTCACTG | NM_001077840.1 | 169 | BCL2 | F: TGGATGACCGAGTACCTGAA R: CAGCCAGGAGAAATCAAACA | NM_001166486.1 | 120 | BAX | F: CACCAAGAAGCTGAGCGAGTGT R: TCGGAAAAAGACCTCTCGGGGA | XM_027515208.1 | 118 | Autophagy-related genes | Beclin-1 | F: AGTTGAGAAAGGCGAGACAC R: GATGGAATAGGAACCACCAC | NM_001033627.2 | 100 | MAP1LC3A (LC3A) | F: CATGAGCGAGTTGGTCAAAA R: GGGAGGCGTAGACCATGTAG | XM_027558753.1 | 170 | MAP1LC3B (LC3B) | F: TTATCCGAGAGCAGCATCC R: AGGCTTGATTAGCATTGAGC | NM_001001169.1 | 170 | ATG7 | F: ATGGCCTTTGAGGAACCTTT R: ATGCCTCCCTTCTGGTTCTT | XM_010817935.3 | 210 | LAMP1 | F: GTGAAGAATGGCAACGGAC R: GCATCAGCTGGACCTCGTAA | XM_027558031.1 | 250 | LAMP2 | F: AAGAGCAGACCGTTTCCGTG R: CGAACACTCTTGGGCAGTAG | XM_027535042.1 | 110 | Cell cycle and DNA damage-related genes | P21 | F: GCAAATATGGGTCTGGGAGA R: AAATAGTCCAGGCCAGGATG | NM_001098958.2 | 112 | P27 | F: TGTCAAACGTGCGAGTGTCTA R: CTCTGCAGTGCTTCTCCAAGT | XM_019961532.1 | 150 | P53 | F: CTATGAGATGTTCCGAGAGC R: CTCTCTCTTGAGCATTGGTT | NM_174201.2 | 153 | NAM methylation-related genes | NNMT | F: CCCAGGTGCTCAAGTGTGAT R: CAGCCTCAAGACACAGGGAG | XM_015474625.2 | 99 | AHCY | F: GCAACTGCTCACTCAGTCCT R: AGGCCTGGATGGTAAAGTGC | NM_001034315.1 | 81 | AOX1 | F: AATGTGACCCGGAAACTCCC R: ATGTGGCCCCCTAAAGAAGC | XM_024999354.1 | 116 | Reference gene | GADPH | F: CCCAGAATATCATCCCTGCT R: CTGCTTCACCACCTTCTTGA | NM_001034034.2 | 185 |
|
|