Elevated LINC00909 Promotes Tumor Progression of Ovarian Cancer via Regulating the miR-23b-3p/MRC2 Axis

Figure 7

LINC00909 achieves its biological functions in ovarian cancer via regulating MRC2. (a) Real-time qPCR revealed mRNA level of MRC2/ZNF839/DEPC1 in SKOV3 cells. In SKOV3 cells, MRC2/ZNF839/DEPC1 was stably knocked down, respectively, using shRNA vector. Scr: scramble. (b) Colony formation assay revealed the tumor characteristics of each SKOV3 cell line. Cells in (a) were used. (c) Real-time qPCR revealed mRNA level of MRC2/ZNF839/DEPC1 in OVCAR4 cells. In OVCAR4 cells, MRC2/ZNF839/DEPC1 was stably overexpressed, respectively. (d) Colony formation assay revealed the tumor characteristics of each OVCAR4 cell line. Cells in (c) were used. (e, f) Colony formation (e) and soft agar (f) assays revealed the tumor characteristics of each OVCAR4 cell line. Cells in S5A were used. (g) Western blot showed the protein level of EMT markers in OVCAR4 cells. Cells in Figure S5A were used. E-cadherin, epithelial marker; N-cadherin/vimentin, mesenchymal marker. (h) The working model of LINC00909 involves the pathogenesis of ovarian cancer cells.

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