Endothelial Cell-Derived SO₂ Controls Endothelial Cell Inflammation, Smooth Muscle Cell Proliferation, and Collagen Synthesis to Inhibit Hypoxic Pulmonary Vascular Remodelling

Figure 2

Increased EC-derived SO₂ ameliorates hypoxia-induced PAEC inflammation, PASMC proliferation, hypertrophy, and collagen production in vivo. (a) Immunofluorescence in situ detection of ICAM-1 protein expression in mouse PAECs; green fluorescence represents ICAM-1 protein. (b) Western blot analysis was performed to detect the expression level of ICAM-1 protein in mouse lung tissue (). (c, d) Immunofluorescence in situ detection of Ki-67, α-SMA, and collagen I protein expression in mouse PASMCs; green fluorescence represents Ki-67 and collagen I protein, and red fluorescence represents α-SMA. The data were expressed as , , scale bar: 20 μm.

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