Vitamin E TPGS 1000 Induces Apoptosis in the K562 Cell Line: Implications for Chronic Myeloid Leukemia

Figure 1

TPGS concentration-dependently induces nuclei fragmentation and loss of mitochondrial membrane potential (ΔΨm) in K562 cells. K562 cells were left untreated (0 μM) or treated with increasing concentrations of TPGS (10–80 μM) at 37°C for 24 h. After this time, cells were double-stained with PI (ex. 535 nm, em. 615 nm) and DiOC₆(3) (ex. 450–490 nm, em. 515 nm) stain. (a) Representative contour plot figures showing alive cells (Q3, PI^low/DiOC₆(3)^high), early apoptotic (EA) cells (Q4, PI^low/DiOC₆(3)^low), late apoptotic (LA) cells (Q1, PI^high/DiOC₆(3)^low), and unspecific cell death, UCD (Q2, PI^high/DiOC₆(3)^high). A: represents the percentage of apoptotic cells (Q1+Q4). The purple number in circles represents viable cells, and the red number in rectangles represents total apoptotic cells. (b) Representative histogram of DiOC₆(3)^low/high and UCD assessed by flow cytometry. (c) Representative histogram of PI^low/high and UCD assessed by flow cytometry according to Materials and Methods.

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