Vitamin E TPGS 1000 Induces Apoptosis in the K562 Cell Line: Implications for Chronic Myeloid Leukemia

Figure 8

TPGS induces activation of effector protein caspase-3 in K562 cells. K562 cells were incubated with TPGS (40–60 μM) at 37°C for 24 h. (a) Protein extracts were blotted with primary antibodies as listed. (b) Relative infrared fluorescence of antibodies was expressed as the fold increase relative to that in untreated cells and normalized to actin. Data are expressed as ; , , and . K562 cells were incubated with increasing concentrations of TPGS (10–80 μM) and NSCI (10 μM) at 37°C for 24 h. After this time, cells were double-stained with PI and DiOC₆(3) stains. (c) Representative contour plot figures showing alive cells (Q3), early apoptotic (EA) cells (Q4), late apoptotic (LA) cells (Q1), and unspecified cell death, UCD (Q2). A: represents the percentage of apoptotic cells as Q1+Q4. The purple number in circles represents viable cells, and the red number in rectangles represents total apoptotic cells. (d) Representative histogram of DiOC₆(3)^low/high and UCD assessed by flow cytometry. (e) Representative histogram of PI^low/high and UCD assessed by flow cytometry according to Materials and Methods.

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