Autophagic Degradation of Gasdermin D Protects against Nucleus Pulposus Cell Pyroptosis and Retards Intervertebral Disc Degeneration In Vivo

Figure 2

LPS induced NP cell pyroptosis in vitro in a dose-dependent manner. (a) Western blot analysis of pro-caspase-1, cleaved caspase-1, GSDMD, GSDMD-N, and NLRP3 in NP cells treated with LPS (0.2 μg/mL, 0.5 μg/mL, and 1 μg/mL) for 24 h. The control group was treated with equivalent solvent. (b) Quantification of cleaved caspase-1, GSDMD-N, and NLRP3 protein levels. GAPDH was used as an internal control. (c) Measurement of LDH activity in LPS-treated NP cells. ELISA analyzed the levels of secreted (d) TNF-α, (e) IL-1β, and (f) IL-6 in LPS-treated NP cells. Data were presented as the , . , , and vs. the control group.

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