Autophagic Degradation of Gasdermin D Protects against Nucleus Pulposus Cell Pyroptosis and Retards Intervertebral Disc Degeneration In Vivo

Figure 7

Administration of VX-765 ameliorated the progression of IDD in vivo. (a) Histological staining, including HE, Alcian blue, and Masson staining, showed the morphology of rat intervertebral disc. The IDD, si-ATG5, and VX-765 groups were treated with needle puncture, while the control group is an untreated group. (b) Histological grades were calculated based on the histological staining results. (c) Immunohistochemistry staining for COL2A1, ATG5, and GSDMD-N was conducted to evaluate the expression level of proteins in tissues. (d) Quantification of COL2A1, ATG5, and GSDMD-N in immunohistochemistry staining. (f) Immunofluorescence staining of caspase-1 and (e) quantification results of the caspase-1 mean fluorescence intensity. and vs. the control group; ns, , no significant difference. Data were presented as the , .

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