Ferritinophagy-Mediated ROS Production Contributed to Proliferation Inhibition, Apoptosis, and Ferroptosis Induction in Action of Mechanism of 2-Pyridylhydrazone Dithiocarbamate Acetate

Figure 4

PdtaA induces ferritinophagy. Nuclei stained by DAPI in blue, ferritin labeled in red, and NCOA4 labeled in green. Red or green fluorescence is due to the secondary antibody attached different fluorescence groups; at given excitation wavelength, the emission of the groups showed either red or green. (a–d) Control (70% DMSO): (a) nuclei, (b) ferritin, (c) NCOA4, and (d) merge of ferritin with NCOA4. (e–h) The PdtaA-treated group: (e) nuclei, (f) ferritin, (g) NCOA4, and (h) merge of ferritin with NCOA4. (i–l) siRNA-mate: (i) nuclei, (j) ferritin, (k) NCOA4, and (l) merge of ferritin with NCOA4. (m–p) PdtaA plus siRNA-mate: (m) nuclei, (n) ferritin, (o) NCOA4, and (p) merge of ferritin with NCOA4. (q–t) PdtaA plus siRNA-NCOA4: (q) nuclei, (r) ferritin, (s) NCOA4, and (t) merge of ferritin with NCOA4. Scale bar, 100 μm.

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