Lead-Induced Motor Dysfunction Is Associated with Oxidative Stress, Proteome Modulation, and Neurodegeneration in Motor Cortex of Rats

Figure 1

Sample description and experimental steps: (a) sample description ( per group) and lead administration protocol. After the administration period to lead acetate, the behavioral evaluation through (1) open field and (2) inclined plane tests (25 animals per group). Then, the euthanasia and brain collection for analyses in motor cortex: (b) oxidative biochemistry analyses through nitrite levels (nitrite) and lipid peroxidation (LPO), with 10 animals per group; (c) proteomic profile performed by mass spectrometry with 9 animals per group; (d) immunohistochemistry analysis by anti-NeuN (NeuN⁺ cells) in M1 area of motor cortex with 6 animals per group.

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