Pink1/PARK2/mROS-Dependent Mitophagy Initiates the Sensitization of Cancer Cells to Radiation

<div>Changes of autophagy, mROS content, and mitochondrial impairment in HeLa and MCF-7 cells. (a) After treatment with 8 Gy IR and NAC (1 mM), representative pictures of autophagy were measured with MDC staining by FCM. (b) Autophagic rates were analyzed by FCM. (c) Western blot analysis of LC3, GAPDH protein was used for loading control, and LC3II/LC3I was quantified. (d) ROS contents were detected by DCFH-DA staining (10 <i>μ</i>M) and analyzed by FCM after 8 Gy IR and NAC (1 mM) treatment. (e) The mitochondrial mass was detected by MitoTracker Green (200 nM) staining. Scale bar, 50 <i>μ</i>m. 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Oxidative Medicine and Cellular Longevity

fig1

Figure 1

Figure 1: Pink1/PARK2/mROS-Dependent Mitophagy Initiates the Sensitization of Cancer Cells to Radiation 