Oxidative Medicine and Cellular Longevity / 2021 / Article / Tab 4 / Research Article
Long-Term Administration of Abacavir and Etravirine Impairs Semen Quality and Alters Redox System and Bone Metabolism in Growing Male Wistar Rats Table 4 Effect of 16-week administration of abacavir and etravirine on semen parameters (AB group: group receiving abacavir 60 mg/kg for 16 weeks; ET group: group receiving etravirine 40 mg/kg for 16 weeks; DFI: DNA fragmentation index; HDS: high DNA stainability. Results presented as median (lower quartile–upper quartile); ^abacavir vs. control group,
; etravirine vs. control group,
).Control group AB group ET group Total sperm count [mln] 58.9 (40.7-87.6) 64.2 (54.4-84.1) 63.8 (42.8-75.6) Sperm concentration [mln/ml] 73.7 (50.8-109.5) 80.3 (68.0-102.1) 79.8 (53.5-94.6) Subjective motility [%] 57.5 (55-65) 62.5 (50-65) 62.5 (52.5-65) Sperm morphology Morphologically normal [%] 93.0 (87.2-93.2) 88.6 (87.2-92.2) 92.9 (83.6-96.1) Proximal droplet [%] 0 (0-0) 0 (0-0) 0 (0-0) Distal droplet [%] 0.75 (0-0.75) 0.62 (0.25-1.50) 0.5 (0.37-0.75) Head abnormalities [%] 0 (0-0) 0 (0-0.25) 0 (0-0.37) Detached head [%] 2.5 (2-3) 2.87 (2.5-3.5) 1.87 (1.0-4.37) Acrosome abnormalities [%] 0 (0-0) 0 (0-0) 0 (0-0) Midpiece defects [%] 2.25 (1-6.2) 5.5 (2.2-6.2) 2.12 (0.87-7.5) “Dag-like” defect [%] 0 (0-0) 0 (0-0) 0 (0-0) Bent tail [%] 2 (1-2.7) 1.6 (1.2-3.0) 1.1 (0.37-3.0) Coiled tail [%] 0 (0-0) 0 (0-0) 0 (0-0) Plasma membrane and acrosome integrity Live cells with intact acrosome [%] 39.7 (33.9-45.2) 42.3 (32.3-44.9) 44.5 (40.8-50.2) Live cells with ruptured acrosome [%] 0.23 (0.1-0.23) 0.17 (0.12-0.31) 0.15 (0.12-0.24) Dead cells with intact acrosome [%] 58.9 (52.3-63.7) 56.0 (53.5-66.5) 54.0 (47.9-57.2) Dead cells with ruptured acrosome [%] 2.09 (1.28-2.19) 1.57 (1.25-2.05) 1.60 (0.87-2.34) Mitochondrial activity High mitochondrial activity [%] 6.54 (5.68-7.85) 3.20 (1.71-5.17) 3.25 (1.94-4.72) Low mitochondrial activity [%] 93.5 (92.1-94.3) 96.8 (94.8-98.3) 96.7 (95.3-98.0) Lipid peroxidation Live without LPO [%] 23.3 (20.0-25.7) 24.7 (22.5-27.3) 24.7 (22.5-27.3) Live with LPO [%] 0.06 (0.03-0.21) 0.05 (0.03-0.16) 0.05 (0.03-0.16) Dead without LPO [%] 75.7 (73.7-78.2) 74.5 (72.1-76.4) 74.5 (72.1-76.4) Dead with LPO [%] 0.79 (0.57-0.82) 0.68 (0.51-1.15) 0.68 (0.51-1.15) Apoptosis and necrosis Live cells [%] 42.6 (31.0-44.2) 48.8 (45.7-51.9) 54.3 (41.0-60.3) Dead cells [%] 55.1 (54.5-65.5) 48.5 (45.4-51.0) 43.3 (38.4-55.2) Apoptotic and necrotic cells [%] 1.64 (1.28-2.09) 1.85 (1.72-2.27) 2.20 (1.33-4.56) Apoptotic cells [%] 0.61 (0.07-0.82) 0.75 (0.12-1.25) 0.06 (0.02-0.12) Chromatin status DFI [%] 0.85 (0.61-1.00) 1.56 (1.18-2.00)^ HDS [%] 18.5 (10.5-20.5) 8.0 (6.5-12.1) 12.1 (11.6-18.6)