Programmed NP Cell Death Induced by Mitochondrial ROS in a One-Strike Loading Disc Degeneration Organ Culture Model

Figure 6

Mitochondrial ROS participated in MS-induced IVD dynamic compressive stiffness reduction and morphological degenerative changes. (a) Representative strain curves of the IVDs during stiffness gauging at a single time point. (b) Representative stress curves of the IVDs during stiffness gauging at a single time point. (c, d) Dynamic compressive stiffness measured on day 1 after overnight swelling (the day after dissection), on day 2 after overnight swelling (the day after MS), and on day 7 after overnight swelling recovery. The data were normalized to the levels on day 1 after overnight swelling. (e) The morphology of the IVDs was evaluated by Safranin O/Fast Green staining. Scale bars, 625 μm. (f) Degeneration scores based on Safranin O/Fast Green staining. The data are expressed as the . , , and .

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