THAP9-AS1 Promotes Tumorigenesis and Reduces ROS Generation through the JAK2/STAT3 Signaling Pathway by Increasing SOCS3 Promoter Methylation in Osteosarcoma
THAP9-AS1 negatively regulated SOCS3 in OS cells and tissues. (a) The mRNA levels of SOCS3 were downregulated in OS cells. (b) The mRNA levels of SOCS3 were restored in OS cells treated with 5-aza. (c, d) Knockdown of DNMTs in MG63 cells increased the mRNA and protein expression of SOCS3. (e) The expression of THAP9-AS1 was effectively downregulated in MG63 cells after transfection with si-THAP9-AS1. (f, g) The mRNA and protein expression of SOCS3 was inhibited by THAP9-AS1 but increased by si-THAP9-AS1. (h) The mRNA levels of SOCS3 were significantly reduced in OS tissues. (i) The mRNA levels of SOCS3 were inversely correlated with THAP9-AS1 in OS tissues. ;. ns: no significance; si-DNMTs: siRNAs for knocking down DNMTs; si-THAP9-AS1: siRNA specific for silencing THAP9-AS1; si-ctrl: siRNA negative control.
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