THAP9-AS1 Promotes Tumorigenesis and Reduces ROS Generation through the JAK2/STAT3 Signaling Pathway by Increasing SOCS3 Promoter Methylation in Osteosarcoma
THAP9-AS1 promoted tumor growth and metastasis in vivo. (a, b) Upregulation of THAP9-AS1 increased the volumes and weights of mouse tumors. (c) The expression of THA9-AS1 in mouse tumors was determined. (d) The expression of SOCS3 was decreased in tumors originating from the THAP9-AS1 group. (e) IHC assay for the expression of Ki-67 and p-STAT3 in mouse tumors; scale bars: 100 μm. (f) The number of lung metastatic nodes was increased by THAP9-AS1; HE staining was conducted to verify the lung metastasis foci; scale bars: 100 μm. (g) A schematic showing that THAP9-AS1 contributed to cell proliferation and metastasis and inhibited ROS production, probably through epigenetically suppressing SOCS3, thereby activating the JAK2/STAT3 signaling pathway. ;.
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