Microglia Polarization from M1 toward M2 Phenotype Is Promoted by Astragalus Polysaccharides Mediated through Inhibition of miR-155 in Experimental Autoimmune Encephalomyelitis

Table 1

Primer sequence used in the experiments.

Gene	Primer sequence
TNF-α	Forward:5-ATGTCTCAGCCTCTTCTCATTC-3
	Reverse:5-GCTTGTCACTCGAATTTTGAGA-3
iNOS	Forward:5-GTT CTC AGC CCA ACA ATA CAA GA-3
	Reverse:5-GTG GAC GGG TCG ATG TCA C-3
IL-12	Forward:5-AGT GAC ATG TGG AAT GGC GT-3
	Reverse:5-CAG TTC AAT GGG CAG GGT CT-3
Arg-1	Forward:5-CAT ATC TGC CAA AGA CAT CGT G-3
	Reverse:5-GAC ATC AAA GCT CAG GTG AAT C-3
CD206	Forward:5-CCT ATG AAA ATT GGG CTT ACG G-3
	Reverse:5-CTG ACA AAT CCA GTT GTT GAG G-3
IL-10	Forward:5-TTCTTTCAAACAAAGGACCAGC-3
	Reverse:5-GCAACCCAAGTAACCCTTAAAG-3
U6	Forward:5-CGCTTCGGCAGCACATATAC-3
	Reverse:5-AAATATGGAACGCTTCACGA-3
miR-155	Forward:5-TGCGCTTAATGCTAATTGTGATA-3
	Reverse:5-CCAGTGCAGGGTCCGAGGTATT-3
IL-1α	Forward:5-CGAAGACTACAGTTCTGCCATT-3
	Reverse:5-GACGTTTCAGAGGTTCTCAGAG-3
C1q	Forward:5-AAAGGCAATCCAGGCAATATCA-3
	Reverse:5-TGGTTCTGGTATGGACTCTCC-3
C3	Forward:5-CCAGCTCCCCATTAGCTCTG-3
	Reverse:5-GCACTTGCCTCTTTAGGAAGTC-3
GFAP	Forward:5-CGGAGACGCATCACCTCTG-3
	Reverse:5-AGGGAGTGGAGGAGTCATTCG-3
GAPDH	Forward:5-AGGTCGGTGTGAACGGATTTG-3
	Reverse:5-TGTAGACCATGTAGTTGAGGTCA-3