SPP1 Promotes Enzalutamide Resistance and Epithelial-Mesenchymal-Transition Activation in Castration-Resistant Prostate Cancer via PI3K/AKT and ERK1/2 Pathways

Figure 6

PI3K/AKT signaling activated by SPP1 in PCa cell lines. In SPP1 siRNA-transfected 22Rv1 cell lines, p-PI3K and p-AKT expression levels were decreased significantly. In contrast, p-PI3K and p-AKT were significantly upregulated in SPP1-overexpressed LNCaP cell line. And SPP1 overexpression was significantly upregulated AR expression in LNCaP cell line. Lentivirus-transfected LNCaP cells were treated with PI3K inhibitor BEZ235 (50 nM) for 48 h. PI3K inhibitor can reverse the effect of SPP1 overexpression on p-AKT and AR expression. ( values were computed by two-tailed unpaired Student’s -tests, , , and .)