Ionizing Radiation Upregulates Glutamine Metabolism and Induces Cell Death via Accumulation of Reactive Oxygen Species

Figure 4

Ionizing radiation induces mitophagy in tumor cells. MCF-7 and HCT-116 cells were irradiated with X-rays at different doses. The acidity of cells was detected by flow cytometry with acridine orange staining (a). The expression of autophagy-associated proteins LC3B and Beclin 1 was increased, while that of p62 was decreased (b). X-rays increased the mitochondrial and lysosome colocalization (c). The expression of mitophagy-associated proteins Parkin and LAMP2 was increased in a dose-dependent manner (d). MCF-7 cells were irradiated with X-rays at different doses, and this induced the increased expression of cytochrome C (e) and very mild apoptosis at 24 h after irradiation (f). of triplicate assessments, one-way ANOVA with post hoc Dunnett test, , , and (a–f).

(a)

(b)

(c)

(d)

(e)

(f)