Deficiency of MIF Accentuates Overloaded Compression-Induced Nucleus Pulposus Cell Oxidative Damage via Depressing Mitophagy

Figure 1

Effects of graded mechanical compression on cell senescence and viability of the human NP cells cultured in a bioreactor: (a) NP cell-encapsulated hydrogels cultured in chambers were dynamically compressed with 0%, 5%, 10%, and 20% deformation for 2 weeks, and the units of our self-developed bioreactor include (1) tissue culture chambers and compression loading application device; (2) substance exchanger peristaltic pumps; (3) medium container; and (4) pH, PO₂, and PCO₂ sensor. (b) The cell senescence degree of the NP cells that exerted graded compression loading was analyzed by SA-β-Gal staining (100x). (c) Statistic analysis of the SA-β-Gal positive rate of the NP cells that exerted graded compression loading. (d) The NP cell viability was analyzed by fluorescent Live/Dead staining (100x). (e) Statistic analysis of the cell Live/Dead rate of the NP cells that exerted graded compression loading. . ^#. .

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