Deficiency of MIF Accentuates Overloaded Compression-Induced Nucleus Pulposus Cell Oxidative Damage via Depressing Mitophagy

Figure 3

Schematic workflow of the TMT quantitative proteomics procedure: NP cell-encapsulated hydrogels cultured under dynamical compression with 0% (control), 5% (low-compression loading), and 20% deformation (high-compression loading) for 2 weeks, and protein extracts were labeled with 10-plex TMT reagent. Peptides were analyzed via LC-MS/MS, and the raw data for protein identification and relative quantification were analyzed by Proteome Discoverer.