miRNA-146a Mimic Inhibits NOX4/P38 Signalling to Ameliorate Mouse Myocardial Ischaemia Reperfusion (I/R) Injury

Figure 1

miR-146a protects NRCMs from H/R-induced inflammation. (a) The mRNA level of miR-146a in heart tissue after I/R injury () and in cardiomyocytes after H/R injury (). (b, c) NRCMs were transfected with the miR-146a mimic and subjected to H/R injury. (b) Cell viability detected by the CKK8 assay and LDH release in each group (). (c) TNFα and IL-1β production in cells detected by the ELISA (). vs. vehicle-CON; ^#vs. vehicle-H/R. (d, e) NRCMs were transfected with the miR-146a inhibitor and subjected to H/R injury. (d) Cell viability detected by the CKK8 assay and LDH release in each group (). (e) TNFα and IL-1β production in cells detected by ELISA (). vs. vehicle-CON; ^#vs. vehicle-H/R. Cell experiments were repeated for 3 times. Unpaired Student’s -test was used in (a); two-way analysis of variance was used in (b)–(e).

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