miRNA-146a Mimic Inhibits NOX4/P38 Signalling to Ameliorate Mouse Myocardial Ischaemia Reperfusion (I/R) Injury

Figure 2

miR-146a protects NRCMs from H/R-induced oxidative stress. (a, b) NRCMs were transfected with the miR-146a mimic and subjected to H/R injury. (a) ROS level detected by the 2,7-dichlorofluorescin diacetate probe and MD level in each group (). (b) MnSOD and NADPH oxidase activity in cells (). vs. vehicle-CON; ^#vs. vehicle-H/R. (c, d) NRCMs were transfected with the miR-146a inhibitor and subjected to H/R injury. (c) ROS level detected by the 2,7-dichlorofluorescin diacetate probe and MD level in each group (). (d) MnSOD and NADPH oxidase activity in cells (). vs. vehicle-CON; ^#vs. vehicle-H/R. Cell experiments were repeated for 3 times. Two-way analysis of variance followed by Tukey’s post hoc test was used.

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