miRNA-146a Mimic Inhibits NOX4/P38 Signalling to Ameliorate Mouse Myocardial Ischaemia Reperfusion (I/R) Injury

Figure 5

miR-146a affects NOX4-P38 signalling. (a) Protein level of NOX2, NOX4, phosphorylated- (P-) P38, and total- (T-) P38 in heart tissue in mice with miR-146a mimic treatment (). (b) Protein level of NOX4, P-P38, and T-P38 in NRCMs transfected with the miR-146a mimic (). (c) mRNA level of NOX2 and NOX4 in NRCMs transfected with the miR-146a mimic (). (d) Luciferase assay in NRCMs transfected with the miR-146a mimic and miR-146a inhibitor (). Cell experiments were repeated for 3 times. vs. vehicle-sham/CON; ^#vs. vehicle-I/R or vehicle-H/R. Two-way analysis of variance was used in (a)–(c). Unpaired Student’s -test was used in (d).

(a)

(b)

(c)

(d)