miRNA-146a Mimic Inhibits NOX4/P38 Signalling to Ameliorate Mouse Myocardial Ischaemia Reperfusion (I/R) Injury

Figure 6

NOX4 silencing abolishes the effect of the miR-146a inhibitor. (a) NRCMs were transfected with NOX4 siRNA (). The protein level of NOX4 after 48 h of transfection (). vs. ScRNA. (b–d) NRCMs were transfected with the miR-146a inhibitor and/or NOX4 siRNA and subjected to H/R injury. (b) Cell viability detected by the CKK8 assay and LDH release in each group (). (c) TNFα and IL-1β production in cells detected by ELISA (). (d) ROS level and MnSOD and NADPH oxidase activity in cells (). vs. ScRNA-CON; ^#vs. ScRNA-H/R. Cell experiments were repeated for 3 times. Unpaired Student’s -test was used in (a); one-way analysis of variance was used in (b)–(d).

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