Oxidative Medicine and Cellular Longevity / 2021 / Article / Fig 2

Research Article

HIF-1α-Mediated miR-623 Regulates Apoptosis and Inflammatory Responses of Nucleus Pulposus Induced by Oxidative Stress via Targeting TXNIP

Figure 2

miR-623 decreases the apoptosis of NP cells induced by oxidative stress. (a) Quantification analysis of miR-623 mRNA expression in NP cells with or without miR-623 mimic transfection. (b) FACS analysis of NP cell apoptosis induced by TBHP with or without miR-623 mimic transfection. (c) The means of cell apoptosis in (b) were shown as the column chart. (d) Western blot analysis of BAX, BCL2, cleaved caspase-3, collagen II, and MMP13 expression in NP cells induced by TBHP with or without miR-623 mimic transfection. (e) FACS analysis of JC-1-positive cells induced by TBHP with or without miR-623 mimic transfection. (f) The means of JC-1-positive cells in (e) were shown as the column chart. (g) FACS analysis of ROS-positive cells induced by TBHP induced with or without miR-623 mimic transfection. (h) The means of ROS-positive cells in (g) were shown as the column chart. (i) FACS analysis of NP cell apoptosis induced by TBHP with or without miR-623 mimic transfection. (j) The means of cell apoptosis in (i) were shown as the column chart. (k) FACS analysis of JC-1-positive cells induced by TBHP with or without miR-623 mimic transfection. (l) The means of JC-1-positive cells in (k) were shown as the column chart. (m) FACS analysis of ROS-positive cells induced by TBHP induced with or without miR-623 mimic transfection. (n) The means of ROS-positive cells in (m) were shown as the column chart. . values were analyzed by two-tailed -tests in (a) and two-way ANOVA in (c, f, h, j, l, and n).
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