Research Article
HIF-1α-Mediated miR-623 Regulates Apoptosis and Inflammatory Responses of Nucleus Pulposus Induced by Oxidative Stress via Targeting TXNIP
Figure 3
miR-623 inhibits the inflammatory responses of NP cells induced by oxidative stress. (a) Western blot analyses of IL-1β and iNOS in NP cells induced by TBHP with or without miR-623 mimic transfection. (b, c) Quantification analysis of IL-1β and IL-6 mRNA expression in NP cells induced by TBHP with or without miR-623 mimic transfection. (d) Quantification analysis of the secretion of IL-6 in culture medium of NP cells induced by TBHP with or without miR-623 mimic transfection. (e) Western blot analyses of IL-1β, iNOS, and cleaved caspase-1 (C-caspase-1) in NP cells induced by TBHP with or without miR-623 inhibitor transfection. (f, g) Quantification analysis of IL-1β and IL-6 mRNA expression in NP cells induced by TBHP with or without miR-623 inhibitor transfection. (h, i) Quantification analysis of the secretion of IL-1β and IL-6 in culture medium of NP cells induced by TBHP with or without miR-623 inhibitor transfection. . values were analyzed by two-way ANOVA.
(a) |
(b) |
(c) |
(d) |
(e) |
(f) |
(g) |
(h) |
(i) |