HIF-1α-Mediated miR-623 Regulates Apoptosis and Inflammatory Responses of Nucleus Pulposus Induced by Oxidative Stress via Targeting TXNIP
miR-623 inhibits TXNIP expression by targeting its 3-UTR. (a) Bioinformatics analysis was performed to determine the predicted downstream targets of miR-623 with the dataset of TargetScan, miRTarBase, and GSE34095. (b) The fold change of relative mRNA expression of TXNIP analyzed by the dataset of GSE63492. (c) Quantification analysis of TXNIP mRNA expression in normal () and degenerated () NP tissues. (d) Schematic representation of the binding between miR-623 and the TXNIP 3-UTR. (e) Relative luciferase activities were analyzed in 293T cells cotransfected with TXNIP 3-UTR (wild-type or mutant) reporter plasmid and miR-623 mimics or miR-623 control. (f, g) qRT-PCR and Western blot analysis of TXNIP expression in NP cells transfected with miR-623 mimics or miR-623 control. and . values were analyzed by two-tailed -tests in (a, b, d, and e).
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